A method of preparing conductive acrylic fibers by adsorbing copper sulfide on acrylic fibers by a single process. The present invention is a method in which a copper sulfate, nickel sulfate, sodium thiosulfate, a pH adjusting liquid, a compound which forms a complex compound with divalent copper ions, and an acrylic fiber are simultaneously introduced into the same reaction tank, followed by heat treatment. The compound forming the coordination compound is added in an amount of 0.1 to 0.5 g/l. The compound which forms a coordination compound with divalent copper ions used in the present invention has good reaction stability and can uniformly adsorb copper sulfide on the surface of the fiber, thereby improving conductivity without impairing the original physical properties of the acrylic fiber.